The PATh PDX facility is a full service program and operates three independent research rooms behind the barrier on the 2^nd floor of the East Campus Research Facility (ECRF). We maintain an animal breeding colony of NSG mice, an experimental procedure room, and an MRI suite.

1. Animal Breeding Colony
   • PATh maintains its own breeding colony of NSG mice
   • Restricted access to maintain integrity/health of the colony
   • PPE required at all times to maintain sterile working environment
   • Dedicated laminar flow stations for cage transfers and handling
   • Currently available strains are NSG and NSG expressing IL6 human transgene
2. Animal Procedure Room
   • PATh has a dedicated biosafety cabinet and anesthesia equipment for PDX mice
   • PATh operates this room following all BSL-2 protocols
   • Animals used in procedure room come from PATh breeding colony to maintain hygienic integrity of all experimental animals
3. MRI Suite
   • Aspect Imaging M3 High Resolution Compact MRI (1T)
   • Specifically designed to image mice
   • MRI is located behind the barrier to prevent spread of infection to NSG mice
   • MRI can scan using T1 or T2 weighted images and can track tumor progression and metastases
   • MRI uses a permanent magnet which allows for minimal servicing and almost 100% uptime