Chemistry (Cobas)
The following reference intervals have been established for the Cobas 501 chemistry analyzer in the Clinical Pathology Laboratory at Cornell University, and went into effect on November 20th 2017. These intervals were derived for serum samples only. The reference intervals for certain analytes are different in heparinized plasma compared to serum (notably potassium, total protein and globulins), but this is species-dependent.
| Analyte | Units | Canine | Feline | Equine | Bovine | Caprine* | Alpaca* |
|---|---|---|---|---|---|---|---|
| Sodium | mEq/L | 143-150 | 149-158 | 134-142 | 134-144 | 143-154 | 149-156 |
| Potassium | mEq/L | 4.1-5.4 | 3.8-5.5 | 2.4-4.8 | 4.0-5.9 | 4.2-6 | 4.2-5.9 |
| Chloride | mEq/L | 106-114 | 111-124 | 95-104 | 92-99 | 101-116 | 105-116 |
| Bicarbonate | mEq/L | 14-24 | 14-20 | 24-31 | 22-30 | 16-26 | 20-32 |
| Anion Gap | mEq/L | 17-27 | 18-29 | 12-19 | 19-26 | 19-27 | 16-22 |
| Urea nitrogen | mg/dL | 9-26 | 17-35 | 10-22 | 7-19 | 10-35 | 11-30 |
| Creatinine | mg/dL | 0.6-1.4 | 0.8-2.1 | 0.8-1.5 | 0.4-0.9 | 0.3-0.8 | 1-2.3 |
| Calcium | mg/dL | 9.4-11.1 | 9.0-11.3 | 10.8-12.9 | 8.9-10.9 | 8.3-10.3 | 8.1-9.9 |
| Phosphate | mg/dL | 2.7-5.4 | 2.6-5.5 | 2.1-4.7 | 4.1-7.3 | 4.5-7.8 | 3.3-7.7 |
| Magnesium | mEq/L | 1.5-2.1 | 1.7-2.2 | 1.2-1.9 | 1.6-2.5 | 1.5-2.2 | 1.7-2.5 |
| Total protein | g/dL | 5.5-7.2 | 6.6-8.4 | 5.4-7.0 | 6.7-8.8 | 6.2-8 | 5.6-7.0 |
| Albumin | g/dL | 3.2-4.1 | 3.2-4.3 | 2.9-3.6 | 3.3-4.3 | 2.9-4.0 | 2.8-4.2 |
| Globulin | g/dL | 1.9-3.7 | 2.9-4.7 | 2.3-3.8 | 2.8-5.4 | 3.0-4.7 | 2.1-3.1 |
| A/G ratio | 0.9-1.9 | 0.8-1.5 | 0.8-1.5 | 0.6-1.6 | 1.0-2.0 | 1.0-2.0 | |
| Glucose | mg/dL | 68-104 | 71-182 | 71-122 | 57-79 | 35-142 | 99-146 |
| ALT | U/L | 17-95 | 28-109 | ||||
| AST | U/L | 18-56 | 17-48 | 222-489 | 54-135 | 62-145 | 119-285 |
| SDH | U/L | 3-13 | 1-11 | 1-6 | 8-48 | 24-63 | 0-6 |
| GLDH | U/L | 1-20 | 0-12 | 2-10 | 14-141 | 4-19 | |
| LDH | U/L | 24-388 | 71-406 | 218-555 | 725-1122 | 116-443 | |
| ALP | U/L | 7-115 | 11-49 | 88-261 | 27-127 | 17-111 | |
| GGT | U/L | 0-8 | 0-2 | 8-33 | 17-54 | 24-64 | 9-35 |
| Total bilirubin | mg/dL | 0-0.2 | 0-0.1 | 0.5-2.1 | 0-0.1 | 0-0.1 | 0-0.1 |
| Direct bilirubin | mg/dL | 0-0.1 | 0-0 | 0.1-0.3 | 0-0 | 0-0 | 0-0 |
| Indirect bilirubin | mg/dL | 0-0.1 | 0-0.1 | 0.3-2.0 | 0-0.1 | 0-0.1 | 0-0.1 |
| Cholesterol | mg/dL | 136-392 | 101-323 | 68-133 | 163-397 | 46-117 | |
| Triglycerides | mg/dL | 23-102 | 30-106 | 14-65 | 10-19 | 9-53 | |
| Amylase | U/L | 322-1310 | 603-2183 | 3-8 | 14-50 | 824-1686 | |
| Lipase DGGR | U/L | 15-228 | 7-23 | 7-16 | 5-13 | ||
| CK | U/L | 64-314 | 74-386 | 171-567 | 88-292 | 116-464 | 35-132 |
| Iron | µg/dL | 97-263 | 59-169 | 95-217 | 64-224 | 102-194 | 86-203 |
| TIBC | µg/dL | 280-489 | 222-423 | 289-535 | 320-490 | 266-408 | 245-441 |
| %Sat | % | 27-66 | 20-56 | 27-56 | 18-54 | 30-59 | 28-59 |
| Bile Acids** | umol/L | 2-10 | 22-247 | ||||
| NEFA | mEq/L | 0.13-1.25 | 0.15-1.05 | 0.02-0.43 | 0.07-0.46 | 0.02-0.27 | |
| BHB | mg/dL | 0-1 | 0-1.0 | 1.0-3.1 | 2.3-10 | ||
| Fructosamine | umol/L | 266-381 | 219-365 | 284-387 | |||
| Uric acid | mg/dL | 0.1-0.4 | 0-0.3 | ||||
| Ionized Calcium (ABL-800 Flex) | mmol/L | 1.16 - 1.40 | 1.11 - 1.38 | 1.40 - 1.72 | 1.16 - 1.33 | - | 1.03-1.32 |
| Cholinesterase | U/L | 3164-8164 | 1540-4055 | 4382-9851 | 119-239 | ||
| SAA+ | ug/mL | 0-8 | |||||
| C-reactive protein++ | mg/L | 0-12 |
* Based on correlations between new and old instruments.
** A standard comment will be provided versus reference intervals.
+ Serum amyloid A in horses: Some normal horses may have values as high as 21 ug/mL.
++ C-reactive protein in dogs: Some normal dogs may have values as high as 22 mg/L.
