Pandemics can impact every person on Earth, causing anguish, loss of life, and economic hardships. Current pandemic mitigation efforts are reactive, focusing on early detection of pathogens (e.g., surveillance) or slowing the spread and impact of pathogens once they are circulating in human populations (e.g., vaccines and therapeutics). We seek to develop a roadmap to proactive pandemic prevention that identifies and addresses the factors that cause pathogens to enter the human population in the first place. We will create a best practices roadmap for spillover investigation and prevention that builds on programs funded by the World Bank. We will communicate our roadmap to the World Bank Pandemic Fund to guide future investments and will develop an online course for communicating best practices to local communities.