Abstract: This is a pilot field observational study to validate the use an owner assessed health related quality of life (HRQL; NewMetrica®) and veterinarian assessed frailty score as healthspan endpoints in age group and size group matched dogs. The primary objective of this pilot cross sectional observational study is to investigate potential differences in HRQL and frailty score across groups. Additionally, we hypothesize that HRQL will show a correlative relationship with frailty score in age group and size group matched dogs. Secondary objectives are to explore relationships with serum IGF1, HRQL, and frailty score. Blood and saliva samples will be collected and banked to perform subsequent epigenetic analysis. Additional serum will be collected for subsequent exploratory metabolomics.