This project is an extension of our current NPB project #19-170 “Validating pathogen nucleic acid extraction from animal feed and feed ingredients”. The goal is to evaluate the extraction methods developed during the NPB proposal in samples collected under field studies conducted by Dr. Scott Dee and Cassie Jones. The two studies collected feed samples (conventional soybean meal, organic soybean meal and complete finishing feed) or environmental samples from feed mills that have been spiked with Porcine Reproductive and Respiratory Virus (PRRSV), Senecavirus A (SVA) or Porcine Epidemic Diarrhea virus (PEDV). We will evaluate the extracrion method using the MagMax Core extraction kit and compare its performance with Magmax Viral DNA/RNA and Indimag Pathogen kit. This will allow us to compare the extraction methods and expand the extraction options to be used across different veterinary diagnostic laboratories.