The primary focus of our NIH award (5 R01 GM105958-07) is to define two redox-signaling events activated in cells under conditions of excess reactive oxygen species. Specifically, we aim to define the changes in the activities of the endoplasmic reticulum (ER) and cytoplasmic cellular Hsp70 molecular chaperones during oxidative stress. Measuring the structure and function of purified versions of the Hsp70 chaperones and their co-factors under excessively oxidizing conditions is essential to our research goals. Isolation of pure recombinant protein for biochemical analysis requires a robust and high-resolution chromatography system. This equipment supplement will be used to purchase a reliable Fast Performance Liquid Chromatography (FPLC) system, which is key for us to complete our experiments.