This work will be performed at the Vet-LIRN and NAHLN affiliated Cornell Animal Health Diagnostic Center, one of the most comprehensive veterinary diagnostic laboratories in the country. Our bacterial WGS services and previous methods development work for Vet-LIRN have supported a number of case investigations and resulted in prominent publications. We are currently supporting one other Vet-LIRN sequencing lab in obtaining ISO:17025 accreditation and will continue to sequence isolates from five additional network labs. The proposed studies will have important impacts for both human and veterinary health and will improve the capabilities of the Vet-LIRN to perform effective surveillance for food-borne pathogens.