Advancing the health and well-being of animals and people


Principal Investigator: Andrew Yen

Contact Information: Email: ay13@cornell.edu; Phone: 607-253-3354
Sponsor: New York State Department of Health-NYSTEM
Grant Number: C026718
Title: Flow Cytometry Core Laboratory to Support Stem Cell Research
Annual Direct Cost: $835,435
Project Period: 08/01/11-07/31/15

DESCRIPTION (provided by applicant): This is an application for support to operate and update a core laboratory resource for fluorescence activated cell sorting and flow cytometry at Cornell University, Ithaca, NY, in order to support stem cell studies. There is a robust growing group of faculty doing stem cell research that uses the current flow cytometry core laboratory to pursue their studies. A critical common component of such studies is the need to isolate stem cells based on molecular markers and other phenotypic characteristics. It is also necessary to characterize these cells and their derivatives resulting from genetic manipulation, treatment with agents such as growth factors and culture conditions. Flow cytometry is an almost unique tool to achieve these experimental objectives that are critical to much stem cell research. It is almost uniquely able to characterize and identify single cells. This is critical because stem cells are frequently rare events and their study requires their isolation and characterization. The group and their needs are expanding. The current facility would thus be better able to support the stem cell research here with lower costs to investigators and shorter scheduling lag time. Accordingly this application seeks support for operation to keep user costs low and facilitate research as well as expanded instrument capabilities to accommodate a broader spectrum of experimental procedures faster. The support sought would thus be for an already operational core laboratory that has technical staffing and direction, strong infrastructure, and administrative management already in place. It is thus a proven entity that now needs support to better serve the needs of stem cell researchers at Cornell.