Contact Information: Email: firstname.lastname@example.org; Phone: 607-253-3102
Sponsor: Grayson-Jockey Club
Grant Number: N/A
Title: Generation of Equine iPS Cells for Regenerative Therapy
Annual Direct Cost: $51,093
Project Period: 04/01/11-03/31/13
DESCRIPTION (provided by applicant): The studies outlined in this proposal are designed to generate and characterize equine induced pluripotent stem (iPS) cells from adult dermal (skin) fibroblasts for future in vivo testing and potential clinical application in the treatment of tendonitis. iPS cells are adult body cells that are reprogrammed by the introduction of specific transcription factors associated with stemness. iPS cells are the only stem cells that are both pluripotent (able to give rise to all cell types) and autogenous (generated from the individual patient). Therefore, they theoretically have the greatest regenerative capacity with no associated risks for immune rejection or disease transmission compared to other stem cell types. We have had consistent success in generating murine iPS cells in our laboratory and will use this experience and similar techniques to generate equine iPS cells. We will first set out to generate equine iPS cells from fetal fibroblasts using the lentiviral vector reprogramming system, which is very efficient and should maximize our chances of success. Once we determine the optimal conditions for reprogramming, we will then move to generating iPS cells from adult dermal fibroblasts using non-viral transduction methods in order to generate iPS cells that are clinically applicable. The equine iPS cells will be then be characterized and tested for pluripotency. If successful, we will be the first to generate equine iPS cells and we will be well positioned to test the regenerative capacity of the iPS cells using an established equine tendon injury model.