Sample preparation

All Runs Require	Negative unstained controls (0.5ml minimum volume)	Compensation controls for each fluor (0.5ml minimum volume)
Acquisition Samples	It is nice to have 0.5ml of sample volume with 1million cells per ml.	Data acquisition runs typically only acquire 10,000 to 30,000 gated events, but still require a minimum 250ul of sample volume.
Sort Runs	Cells should be at 20-30 million cells/ml (5,000 to 35,000 events/s)	The Aria can sort at up to 50- 100 million cells/hour (a flow rate of 0.2-0.8ul/s and up to 2ul/s with less resolution)
Sort Time	~1-2 ml of sample volume per hour. Collection rate(cells/hour) = 2(%Viability) X (%Positives) X (cell concentration)	Collection time = (Sort number target) X (collection rate) * not including set-up
Collection Tubes	Investigator should bring collection tubes	1ml, 5ml, or 15ml tubes- some cells require tubes to be coated with BSA/Serum or sorted into tubes containing media
Collection Tubes	1ml microtubes-Bio-Rad-223-9391 5ml (12X75mm)-BD Biosciences-352058 15ml conical-BD Biosciences-352196	Multiwell plates and glass slides 96,48,12,6 well plates max 1.5cm in height slides should be coated
Questions	Please direct all questions to Lavanya at (607)253-4004 biomedflowcore@cornell.edu
Directions	The facility is located in the basement of the Vet. Research Tower down the far right corridor and around the left corner.